EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)

LOCUS       EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)               11683 bp ds-DNA     circular SYN 26-OCT-2022
DEFINITION  EK0285 in plant-expressable format: Sensor 1 (for EK0208); mCherry 
            marker, EGFP output..
ACCESSION   .
VERSION     .
KEYWORDS    EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11683)
  AUTHORS   Kaseniit E, Gao X
  TITLE     RADAR plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 11683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 26, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11683
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        270..315
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             833..1540
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(978..996)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1246..1265
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1577..1642
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             1778..1843
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             1844..2557
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     terminator      2810..3062
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3194..3539
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    4166..4342
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4908..5087
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4940..5123
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5144..5938
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5198..5217)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5808..5827
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6324..6576
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6667..6691
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      7059..7690
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(7780..8928)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9227..10021)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(10611..10628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10862..10881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11313..11337
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11476..11495
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11620..11642
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11634..11651
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11635..11651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgagaa gatagtggaa
       61 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
      121 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
      181 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
      241 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
      301 tttcatttgg agaggtatta aaatcttaat aggttttgat aaaagcgaac gtggggaaac
      361 ccgaaccaaa ccttcttcta aactctctct catctctctt aaagcaaact tctctcttgt
      421 ctttcttgcg tgagcgatct tcaacgttgt cagatcgtgc ttcggcacca gtacaacgtt
      481 ttctttcact gaagcgaaat caaagatctc tttgtggaca cgtagtgcgg cgccattaaa
      541 taacgtgtac ttgtcctatt cttgtcggtg tggtcttggg aaaagaaagc ttgctggagg
      601 ctgctgttca gccccataca ttacttgtta cgattctgct gactttcggc gggtgcaata
      661 tctctacttc tgcttgacga ggtattgttg cctgtacttc tttcttcttc ttcttgctga
      721 ttggttctat aagaaatcta gtattttctt tgaaacagag ttttcccgtg gttttcgaac
      781 ttggagaaag attgttaagc ttctgtatat tctgcccaaa ttcgcgaccg gtatggtgag
      841 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
      901 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      961 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     1021 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     1081 cattcccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     1141 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     1201 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     1261 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     1321 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     1381 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     1441 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     1501 gggccgccac tccaccggcg gcatggacga gctgtacaag agcgaagcca gacacaaaca
     1561 gaaaattgtg gctccggtga aacagacttt gaattttgac cttctgaagc tggcgggaga
     1621 cgtcgagtcc aaccccggtc ccggatccac caagtcgaca cggatgagtg tttccttcta
     1681 tccgcgtcat tgttaggcct tacgtaccga tagaccactg gtgcaaatag agagctatac
     1741 cggtgaggca cggcataagc aaaagatcgt agccccagta aagcaaacac tcaacttcga
     1801 tctactcaaa ctcgcaggtg atgtggaatc taatccagga cctgtctcaa agggcgagga
     1861 actttttacg ggcgttgttc cgatcctcgt cgaattggac ggggacgtta atgggcataa
     1921 attctcagtt agtggtgagg gtgaggggga cgcgacatat gggaaactta cgctgaagtt
     1981 catatgtacc accggaaaat tgccagtgcc gtggcctact ctcgtcacca ccctgacata
     2041 tggtgttcag tgttttagcc ggtatccaga ccatatgaaa cagcacgatt tttttaaatc
     2101 cgctatgcct gaaggttacg tacaggagcg aacgatattt tttaaagacg acggaaacta
     2161 caaaactaga gccgaggtaa aattcgaggg tgacactctc gtgaaccgga tcgaactgaa
     2221 gggcatagat tttaaggagg atggcaatat actgggccac aagctcgaat acaactataa
     2281 ttctcataat gtctatatca tggcggacaa acagaaaaac ggcatcaagg taaactttaa
     2341 aatccgacac aatattgagg acggctctgt ccagcttgcg gaccattacc aacagaacac
     2401 gccgataggg gatggtccag tacttctccc tgataatcac tatttgtcta ctcagtcagc
     2461 tcttagcaag gacccaaacg aaaaacggga tcacatggtc cttcttgagt tcgtcactgc
     2521 ggctgggata acactcggga tggacgaact gtataagtag ctcgaggcct ttaactctgg
     2581 tttcattaaa ttttctttag tttgaattta ctgttattcg gtgtgcattt ctatgtttgg
     2641 tgagcggttt tctgtgctca gagtgtgttt attttatgta atttaatttc tttgtgagct
     2701 cctgtttagc aggtcgtccc ttcagcaagg acacaaaaag attttaattt tattaaaaaa
     2761 aaaaaaaaaa aaaagaccgg gaattcgata tcaagcttat cgacctgcag atcgttcaaa
     2821 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     2881 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     2941 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     3001 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     3061 tctctagagt ctcaagcttg gcgcgccagc ttggcgtaat catggtcata gctgttgcga
     3121 ttaagaattc gagctcggta cccccctact ccaaaaatgt caaagataca gtctcagaag
     3181 accaaagggc tattgagact tttcaacaaa gggtaatttc gggaaacctc ctcggattcc
     3241 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca
     3301 aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tgcctctgcc gacagtggtc
     3361 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     3421 cttcaaagca agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc
     3481 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag
     3541 cccaagcttc gactctagag gatcccctta aatcgatatg gaacgagcta tacaaggaaa
     3601 cgacgctagg gaacaagcta acagtgaacg ttgggatgga ggatcaggag gtaccacttc
     3661 tcccttcaaa cttcctgacg aaagtccgag ttggactgag tggcggctac ataacgatga
     3721 gacgaattcg aatcaagata atccccttgg tttcaaggaa agctggggtt tcgggaaagt
     3781 tgtatttaag agatatctca gatacgacag gacggaagct tcactgcaca gagtccttgg
     3841 atcttggacg ggagattcgg ttaactatgc agcatctcga tttttcggtt tcgaccagat
     3901 cggatgtacc tatagtattc ggtttcgagg agttagtatc accgtttctg gagggtctcg
     3961 aactcttcag catctctgtg agatggcaat tcggtctaag caagaactgc tacagcttgc
     4021 cccaatcgaa gtggaaagta atgtatcaag aggatgccct gaaggtactc aaaccttcga
     4081 aaaagaaagc gagtaagtcg agggcgagct cgaattcggt acgctgaaat caccagtctc
     4141 tctctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat
     4201 aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     4261 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     4321 atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt gaatataaac
     4381 cagacacgag acgactaaac ctggagccca gacgccgttc gaagctagaa gtaccgctta
     4441 ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga ctcccccgta
     4501 ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg aaggataagg
     4561 ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct actatactta
     4621 tactatacgc taagggaatg cttgtattta taccctatac cccctaataa ccccttatca
     4681 atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc catgaatagg
     4741 tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt tcttaactct
     4801 aaagataaaa gatggcgcgt ggccggccat gagcggagaa ttaagggagt cacgttatga
     4861 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt
     4921 gaaggagcca ctcagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     4981 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     5041 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     5101 actctcaatc caaataatct gcaccggatc tggatcgttt cgcatgattg aacaagatgg
     5161 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5221 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5281 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     5341 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5401 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5461 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5521 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5581 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5641 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt
     5701 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5761 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5821 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5881 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5941 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6001 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6061 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccacgg gatctctgcg
     6121 gaacaggcgg tcgaaggtgc cgatatcatt acgacagcaa cggccgacaa gcacaacgcc
     6181 acgatcctga gcgacaatat gatcgcggcg tccacatcaa cggcgtcggc ggcgactgcc
     6241 caggcaagac cgagatgcac cgcgatatct tgctgcgttc ggatattttc gtggagttcc
     6301 cgccacagac ccggatgatc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     6361 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     6421 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     6481 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     6541 aattatcgcg cgcggtgtca tctatgttac tagatcgggg gccggccctc actggtgaaa
     6601 agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     6661 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     6721 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     6781 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     6841 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
     6901 cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta
     6961 tcagccttct tattcatttc tcgcttaacc gtgacagagt agacaggctg tctcgcggcc
     7021 gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga
     7081 agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa
     7141 aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa
     7201 aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt
     7261 caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc
     7321 tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct
     7381 tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc
     7441 tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc
     7501 gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt
     7561 ttccgcgagg tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt
     7621 ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc
     7681 ggtgagcgtc ggaaaggcgc tcggtcttgc cttgctcgtc ggtgatgtac actagtcgct
     7741 ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag
     7801 gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc
     7861 gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa
     7921 ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg
     7981 ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc
     8041 aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg
     8101 gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga
     8161 cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata
     8221 ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg
     8281 ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc
     8341 gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat
     8401 gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga
     8461 gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa
     8521 ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc
     8581 ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt
     8641 tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg
     8701 cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc
     8761 gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg
     8821 gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc
     8881 gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg
     8941 cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg
     9001 tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg
     9061 gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt
     9121 gcccaaatac ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc
     9181 ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa aacaattcat
     9241 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     9301 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
     9361 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
     9421 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
     9481 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
     9541 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
     9601 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
     9661 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
     9721 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
     9781 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
     9841 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
     9901 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
     9961 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
    10021 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
    10081 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
    10141 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
    10201 accacaatta tgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
    10261 tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt
    10321 gcgtaacggc aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac
    10381 atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga
    10441 tatggccatg aatggcgttg gatgccgggc aacagcccgc attatgggcg ttggcctcaa
    10501 cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg taactatgcg gtgtgaaata
    10561 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
    10621 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10681 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10741 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10801 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10861 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10921 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10981 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    11041 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    11101 ccggtaagac acgacttatc gccactggca gcaggtaacc tcgcgcatac agccgggcag
    11161 tgacgtcatc gtctgcgcgg aaatggacgg gcccccggcg ccagatctgg ggaaccctgt
    11221 ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc
    11281 cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
    11341 actgatagtt tgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc
    11401 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
    11461 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gccattcagg ctgcgcaact
    11521 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    11581 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    11641 cgacggccag tgaattgtta attaagaatt cgagctccac cgc
//